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Bacto Laboratories bacto peptone
Bacto Peptone, supplied by Bacto Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bacto-peptone/bacto+extract+yeast/pm42173498-86-13-13
Average 86 stars, based on 1 article reviews
bacto peptone - by Bioz Stars, 2026-10
86/100 stars

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Suspension:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Incubation:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Modification:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Quantitative RT-PCR:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Control:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Expressing:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Sequencing:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.

Construct:

Article Title: Functional study of FleQ reveals the co-regulation of flgG by rpoN2vemRfleQ operon members in Xanthomonas citri subsp. citri
Article Snippet: A cell suspension of each Xcc strain was adjusted to OD 600 =0.2 and then stabbed into 0.3% agar plates composed of 1.0% Bacto-Peptone and 0.5% NaCl followed by incubation at 28°C for 4 d. The diameter of the area occupied by each strain was measured, and the values were used to indicate the motility of Xcc strains.

Article Title: Optimizing the treatment and valorization of spent coffee grounds (SCGs) through the integration of sequential pretreatment steps, enzymatic hydrolysis, and selective fermentation
Article Snippet: For pre-cultivation, YP medium (10 g/L yeast extract and 20 g/L Bacto-peptone, pH 6.7) with 20 g/L glucose (YPD20) was used.

Article Title: Body-to-brain insulin and Notch signaling regulates memory through neuronal CREB activity
Article Snippet: For all experiments, worms were maintained at 20 °C on plates made from nematode growth medium (NGM: 3 g l −1 NaCl, 2.5 g l −1 Bacto-peptone, 17 g l −1 Bacto-agar in distilled water, with 1 ml l −1 cholesterol (5 mg ml −1 in ethanol), 1 ml l −1 1 M CaCl 2 , 1 ml l −1 1 M MgSO 4 and 25 ml l −1 1 M potassium phosphate buffer (pH 6.0) added to molten agar after autoclaving) or high growth medium (HGM: NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM), with OP50 Escherichia coli for ad libitum feeding.

Article Title: The stn1 - sz2 Mutant Provides New Insight into the Impacts of Telomeric Cdc13-Stn1-Ten1 Dysfunction on Cell Cycle Progression.
Article Snippet: Yeast cultures were grown at the indicated temperatures in YEP (1% yeast extract, 2% bacto-peptone, 0.005% adenine, and 0.005% uracile) supplemented with 2% glucose (YEPD) or in selective minimal media.

Article Title: The GATA-like transcription factor Gat201 determines alkaline-restricted growth in Cryptococcus neoformans .
Article Snippet: Cells from a single colony were inoculated into 25 mL liquid YPDA (1% yeast extract, 2% Bacto-peptone, 2% dextrose, 0.002% adenine) and incubated for 5 days at 150 rpm, 30°C.

Article Title: Methods of preparing personalized blood vessels
Article Snippet: In certain embodiments, the suspension further comprises one or more of: D-Glucose, Phenol red, HEPES, Sodium pyruvate, Glutathione (reduced), Hypoxantine·Na, Thymidine, Lipoic acid, Putrescine 2HCl, Bacto-peptone, Thymine, Adenine sulphate, Adenosine-5-triphosphate, Cholesterol, 2-deoxy-D-ribose, Adenosine-5-phosphate, Guanine HCl, Ribose, Sodium acetate, Tween 80, Uracil, and/or Xanthine Na.

Article Title: A lysosomal surveillance response to stress extends healthspan
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l −1 Bacto-peptone, 30 g l −1 Bacto-agar and 4 ml l −1 cholesterol (5 mg ml −1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source .

Article Title: A lysosomal surveillance response to stress extends healthspan.
Article Snippet: All worm strains were routinely maintained at 20 °C (except for the CB1370 [daf-2(e1370)] strain, which was maintained at 15 °C; the SM190 [pha-4(zu225);smg-1(cc546ts)] strain, which was maintained at 25 °C) on NGM or high growth medium (NGM recipe modified as follows: 20 g l−1 Bacto-peptone, 30 g l−1 Bacto-agar and 4 ml l−1 cholesterol (5 mg ml−1 in ethanol); all other components same as NGM) plates, with Escherichia coli OP50 as the food source93.



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